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CellScrew® cultivation data & documents

Explore the core of CellScrew® with application notes (AN), brochures, and technical data sheets. Find specifications, insights for optimized cell cultivation, and performance data, such as CHO-K1 cell densities of 125,000 cells/cm² and kLa values.
More documents in our library

Differentiated macrophages in CellScrew® mini and 6K

CSm Macrophages IL1b
IL-1β gene expression in response to LPS stimulation (dosed in dependent inflammatory response. BRP-standardized EU/mL). NAT-MAT® Cells seeded at 1 × 10⁴ cells/well
following differentiation in CellScrew® mini; dPCR readout expressed as
copies/μL normalized to HKG; R² = 0.9812.

Macrophage production for MAT assay application

New customer data

The first external evaluation of macrophage production in CellScrew® conducted by Minerva Biolabs.
Human monocytes differentiated into functional NAT-MAT® Cells could be harvested and tested for across two scales, CellScrew® mini and 6K. Differentiation and pyrogen response assessed independently, with IL-1β and TNF-α induction confirmed by dPCR for MAT applications.
Download Application Note

Harvest of B16-F10 and
CT-26 in the CellScrew® mini

Harvest efficiencies and viability of adherent cells cultivated in CellScrew® and harvested with Accutase
Reduction and replacement of dissociation reagent on PLA surface. Harvest efficiency [%] and cell viability [%] of B16-F10 (A) and CT-26 WT (B) cell lines after 96 h of culture on PLA surface in the CellScrew® mini. Cells were detached using 100% Accutase, 50% Accutase + 50% DPBS, or 100% Envzyme Super under identical conditions (n = 1)

Characterization of novel bioreactor

New peer-reviewed publication

The CellScrew® supports robust adherent growth across cell types — HEK-293, B16-F10, and CT-26 WT — while reducing vessel count, lowering reagent use, and minimizing handling steps. A peer-reviewed evaluation of hydrodynamics, oxygen transfer, cell attachment, proliferation, harvest performance, and metabolite profiles across manufacturing-relevant conditions.
Download Research Paper

Harvest of recombinant protein in 51-day culture period of HEK-293

Protein and harvest volume of recombinant protein production in HEK-293 using CellScrew

Recombinant protein production in HEK-293

Long-term recombinant protein production in stably transfected HEK-293 cells cultured in the CellScrew® mini over a 51-day period, demonstrating consistent protein concentration and sustained metabolic activity with zero contamination events. This study establishes the CellScrew® mini as a robust and reliable platform for long-term protein production, from process development through to clinical manufacturing.
Download Poster

Cultivation of CHO-K1 in the CellScrew® mini

CHO-K1 cell density graph in CellScrew® mini over 3 days. CellScrew® cultivation data

Growing adherent CHO-K1 in the CellScrew® mini

Cultivation of CHO-K1 cells on the CellScrew® PLA surface enables expected growth and performance comparable to standard 2D culture systems while maintaining established cell characteristics.
  • expected cell density of 125,000 cells/cm² reached after 3 days
  • comparable CHO-K1 cell yields to standard 2D culture systems
  • retained cell characteristics consistent with PS-based workflows
Download AN – CHO-K1 cultivation

Application of PLA as a growth surface for adherent cells

The sustainable plant-based growth surface shows cell attachment and growth comparable to standard polystyrene surfaces.
  • successful expansion, exceeding DSMZ data
  • high harvesting efficiency with reduced enzyme concentration
Download AN-HEK293 on PLA

Cultivation and Expansion of HEK-293 cells in the CellScrew® 6K

cell density graph

Comparison of handling time of the CellScrew® 6K and competitors

Handling time Figure

Reduction of manual handling time

The efficient design of the CellScrew® enhances processes and results in a reduced handling time compared to competitors, independently of the operator.
  • comparable handling times across operators
  • reduced handling time
  • enhanced process leading to reduced working time
Download AN-Handling

Process Engineering Characterization of the CellScrew® 6K and 10K

5493ed 80ba8b8c61f64eb38e47542c1571ee07mv2

Mixing time and oxygen transfer coefficient

Comparison of mixing time and oxygen transfer coefficient (kLa-value) at different rotational speeds for the CellScrew®.
  • Short mixing times
  • Comparable kLa value to benchtop bioreactor system
Download AN-Characterization

There’s more to discover!

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