HEK-293 Seed Train Expansion with CellScrew®

HEK-293 seed train, transformed.

Minimizing the operational footprint of HEK cell production with the CellScrew® system

Simplifying seed-train production

CellScrew® addresses this directly by consolidating multiple expansion stages into a single vessel format that scales without format change, and replacing multiple stacks and roller bottles.

HEK seed train workflow using CellScrew®

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CellScrew® advantage: dynamic movement and miniaturization

CellScrew® is designed to specifically address the limitations of static, planar culture systems by combining a massive surface area with dynamic cultivation. This unique combination simplifies the workflow, improves gas exchange, and miniaturizes the overall seed train process. For HEK-293 seed trains specifically, this matters because AAV and lentiviral vector titres are sensitive to culture conditions, as inconsistent gas exchange or uneven cell distribution across a vessel can directly affect downstream productivity.

Free up space in your incubator

By combining a high surface area with dynamic operation, CellScrew® reduces handling time and labor by approximately 30% compared to multilayer stack workflows and by up to 92% compared to roller bottle processes, while reducing incubator footprint.

These benefits translate directly into fewer vessels to track, fewer media exchange steps per passage, and a smaller biosafety cabinet or laminar flow workload per production cycle — reducing both the time and contamination risk associated with open-vessel handling at scale.

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